seeker
is an R package for fetching and processing sequencing data, especially RNA-seq data, as well as microarray data. Hopefully it helps you get what you’re after, before the day you die. For more details, see Schoenbachler and Hughey (2022).
Installation
To use seeker
, you can install seeker
and its dependencies, or use a pre-built Docker image in which seeker
and its dependencies are already installed.
R package and dependencies
-
Install the
BiocManager
R package.if (!requireNamespace('BiocManager', quietly = TRUE)) install.packages('BiocManager')
-
Install the
seeker
R package, either from CRAN or from the Hughey Lab drat repository. We useBiocManager::install()
in order to smoothly installseeker
’s dependencies that are on Bioconductor.BiocManager::install('seeker') # CRAN # BiocManager::install('seeker', site_repository = 'https://hugheylab.github.io/drat/') # drat
-
Install the system dependencies to fetch and process sequencing data. The simplest way to do this is to use the function
installSysDeps()
. For example,seeker::installSysDeps('~', '~', '~', '~')
You can also use
installSysDeps()
to fetch genomes from refgenie, such as those required to quantify transcript abundances using salmon.
Docker image
The Docker image is called socker and is based on rocker/rstudio.
Usage
For an introduction to the package, read the vignette. For more details, check out the reference documentation.